The fluorescent dye NILE Red had originally been used for staining of hydrophobic compounds like lipids. In the last years, NILE Red has been tested and proposed for the staining of microplastic particles of polymers like PE,PET,PS etc. I was wondering what interaction leads to the intercalation of NILE Red with those carbon based polymers on the molecular level?
There are parts to the use of Nile Red. First, it is a relatively non-polar substance that prefers non-polar environments such as lipids and most polymers, so it will tend to distribute itself into the non-polar environment rather than polar water. Second, the fluorescent properties of the Nile Red change with environmnet. In polar solvents like water, Nile Red does not fluoresce while in non-polar lipids and polymers, there is a strong red to yellow fluorescence. The wavelength of the absorbance and fluorescence spectra also change with polarity.
Are you sure that Nile Red intercalates, as opposed to just binding to the surface through hydrophobic interactions?
Hello
how we can solve the problem of dying PETE with nile red. it seems there ic no foursent feedback of pete dyed with nile red seen under microscope
A bit outdated, but here is another way of oxidizing organic matter ) Could be also useful Article An efficient and gentle enzymatic digestion protocol for the...
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