Normal interaction and altered interaction with other proteins ,and name of the proteins which is interacting with in cancer cells
Can anyone tell me a rough protein final concentration for a good start? I am using 20fmol biotinylated DNA in 20ul reaction volume. What can be good amount of DNA binding protein to start with?
11 December 2016 5,624 3 View
Can any one provide me the complete sequence of pGWB3 plant expression vector?
04 May 2015 10,144 4 View
I have noticed that my reaction volume has been decreased by 2-3 ul after the restriction digestion of plasmids with different restriction enzymes at 37 °C. I am sure that there is no or very...
07 August 2014 8,367 4 View
I want to design real time PCR primers for my gene of interest which is 1Kb in size and with one intron. What are the important parameters that I should keep in mind while designing primers? Is...
06 July 2014 3,516 8 View
My protein of interest (human) contains glycosylation and having 34 kDa molecular weight. For crystallization purpose.
10 November 2013 2,976 1 View
I want to silence an oncogene (which is transfected) for a period of time and activate it. How can I do that? Could you suggest some papers for the above case,if any? This is a general case.
10 November 2013 3,791 1 View
I would like to know which sequence (shRNA, siRNA etc) is appropriate for gene silencing in plant species? What are the factors that should be considered for selection of RNA sequence?
10 November 2013 8,733 2 View
I am specifically interested in plant hormones which are either only secreted at the time of fruit formation or which are down regulated at that time. No need of specificity, can be any plant.
10 November 2013 10,032 12 View
I would like to know more on their advantages, disadvantage and their use in gene expression analysis.
10 November 2013 7,372 0 View
I want to check the gene expressions of two family of plants. What are the methods that I can use? I also want to know the limitations. A discussion on this topic is expected.
10 November 2013 2,508 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...
03 August 2024 5,828 4 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View