Error prone PCR is one of the methods for random mutagenesis in vitro. The main idea is to use non-high-fidelity DNA polymerase, such as Taq, and introduce 'sloppy' reaction conditions, e.g. by adding Mg2+ or Mn2+. Under these these 'sloppy' conditions Taq will introduce even more errors during synthesis. By playing with conditions and the number of cycles, one can achieve desired level of mutagenesis.

For protocols and more info just Google. Here is very nice protocol: https://molbio.mgh.harvard.edu/szostakweb/protocols/pcr/mutpcr.html

There are also protocols in Protocol Books: http://link.springer.com/protocol/10.1007%2F978-1-60761-652-8_7

and http://link.springer.com/protocol/10.1385%2F1-59259-395-X%3A3

Good Luck!

Thanks Olga!

Please see the papers for your reference

https://www.researchgate.net/publication/311668087_Improvement_of_chitinase_Pachi_with_nematicidal_activities_by_random_mutagenesis

https://www.researchgate.net/publication/289367579_Characterization_and_directed_evolution_of_BliGO_a_novel_glycine_oxidase_from_Bacillus_licheniformis

https://www.researchgate.net/publication/282153514_Enhanced_nematicidal_potential_of_the_chitinase_pachi_from_Pseudomonas_aeruginosa_in_association_with_Cry21Aa

https://www.researchgate.net/publication/277326677_Improvement_of_glycine_oxidase_by_DNA_shuffling_and_site-saturation_mutagenesis_of_F247_residue

Regards

Article Improvement of chitinase Pachi with nematicidal activities b...

Article Characterization and directed evolution of BliGO, a novel gl...

Article Enhanced nematicidal potential of the chitinase pachi from P...

Article Improvement of glycine oxidase by DNA shuffling, and site-sa...