Abhishek Saxena

4 Questions 9 Answers 0 Followers

Questions related from Abhishek Saxena

What do the series matrix files signify in GEO data? Where do I get the raw data for meaningful re-analysis/meta analysis?

It gives GSMxxxx and then lists some values? What do hose values signify? How do I get the .CEL files and are the series matrix data and tabulated data of the individual sample (GSMxxxx) amenable...

15 January 2016 8,932 1 View

Can anybody suggest a suitable vector system for preparing genomic DNA library from yeast Debaryomyces hansenii?

I am interested in making a library of genomic DNA fragments of D. hansenii in a suitable vector that can be propagated in E. coli but can preferably be used as an expression vector in S....

19 December 2014 4,248 1 View

Is there a method for optimising taq pol activity in PCR reaction?

I want to ensure that there is a 1.7X to 1.9X increase in the template per cycle. Is it fine if I set up an identical reaction in 30 tubes and after each cycle keep on removing one tube from the...

14 November 2014 3,024 5 View

Can some one help with error prone PCR?

I want to create mutant libraries for some yeast proteins using error prone PCR. Please share some protocols and also explain the principle of how this method works. Including pros and cons of the...

29 October 2014 6,400 3 View