I'm trying to make Nanostructured lipid carriers (NLC) using ultrasonicator method for cancer meds that are hydrophobic and insoluble in water. I'm heating solid and liquid lipid phase and then adding meds to it and then slowly adding aqueous phase to it which consists of water and poly 80.
Then I sonicate this mixture in ultrasound sonicator to generate nanoparticles.
My question is, does reducing size and encapsulation in lipid increase absorption and bioavailability against cancer cells? I'm not using any solvents. Do I need to? Shouldn't the insoluble drug essentially become soluble once it's particle size is reduced by ultrasonicator ?
Please help. Thanks a lot.
Dear all, solubility depends on the chemical structure and types of existing intermolecular forces. Excessive sonication may degrade any of the compounds present in the recipe. There are many techniques to solubilize poorly soluble drugs. Please check the following links. My Regards
https://www.researchgate.net/publication/236177023_Techniques_for_solubility_enhancement_of_poorly_soluble_drugs_An_overview
https://www.researchgate.net/publication/277233903_Techniques_to_enhance_solubility_of_poorly_soluble_drugs_A_review
https://doi.org/10.5402/2012/195727
Particle size reduction does not increase the equilibrium solubility, only the dissolution rate. There are methods to stabilize the metastable supersaturated solutions, but, upon stability studies, they might precipitate out. It is a false sense that the measured solubility is higher after particle size reduction due to the the method to determine the concentration in the liquid. Centrifugation and/or filtration can leave small particle in the supernatant that, upon sample preparation (for HPLC) can re-dissolve in the solvent used, often a mixture of aqueous and organic components. However, the absorption can be enhanced due to the particle size of the compound that can pass through the biological membrane.
Dear all, solubility depends on the chemical structure and types of existing intermolecular forces. Excessive sonication may degrade any of the compounds present in the recipe. There are many techniques to solubilize poorly soluble drugs. Please check the following links. My Regards
https://www.researchgate.net/publication/236177023_Techniques_for_solubility_enhancement_of_poorly_soluble_drugs_An_overview
https://www.researchgate.net/publication/277233903_Techniques_to_enhance_solubility_of_poorly_soluble_drugs_A_review
https://doi.org/10.5402/2012/195727
Hi;
The decrease in particle size leads to an increase in the contact surface, this effect is very important when the size goes down to the nanometric range. In most cases, a low dissolution rate is correlated with low solubility. Among the techniques for increasing solubility, ultrasonic cavitation is an almost universal method to achieve faster dissolution and better bioavailability.
With my best regards
Abdelkader BOUAZIZ : What is excessive sonication ? Is there a time/temperature/power limit that defines excessive sonication ?
Also, I am currently not using sonication. I tried using DMSO but because of lack of having a freeze dryer I am not able to remove it from my solution. Are there any other solvents that are easy to remove that you would recommend ?
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