I have discovered that rat-specific research assay kits are ELISA kits designed to be used with microplate readers.
Are there no such kits that can be used with spectrophotometers?
Is there any possible explanation of a plant extract having a good in vitro antioxidant activity but poor in vivo protective activity or probably toxic in vivo? Any relevant article is highly...
03 April 2019 3,715 3 View
Is it possible for a drug to have little or no toxic effect on the histology of a particular organ, say the liver but a significant negative effect on same organ from the results of Biochemical...
03 April 2019 1,698 5 View
Most cold extraction of plant samples by maceration are reportedly done for 72 hours (3days). Is there any SIGNIFICANT consequence of leaving it a beat longer say 5days or 6days? Would the active...
07 August 2018 2,935 3 View
When a liver organ is extracted from an experimental animal, they are quickly washed in ice cold 1.15% KCl solution.Why is it always KCl? Can't normal saline be used also?
04 May 2018 6,301 1 View
Can I store pulverized and completely dry leaf samples up to one year before extraction?
03 April 2018 6,409 4 View
I want to know how i can concentrate n-hexane plant extract and subsequently dry it so the shelf life can be extended.
31 December 2016 6,233 7 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...
01 March 2021 6,999 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View
I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.
28 February 2021 6,512 3 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.
28 February 2021 5,929 2 View