I have discovered that rat-specific research assay kits are ELISA kits designed to be used with microplate readers.
Are there no such kits that can be used with spectrophotometers?
Is there any possible explanation of a plant extract having a good in vitro antioxidant activity but poor in vivo protective activity or probably toxic in vivo? Any relevant article is highly...
03 April 2019 3,833 3 View
Is it possible for a drug to have little or no toxic effect on the histology of a particular organ, say the liver but a significant negative effect on same organ from the results of Biochemical...
03 April 2019 1,796 5 View
Most cold extraction of plant samples by maceration are reportedly done for 72 hours (3days). Is there any SIGNIFICANT consequence of leaving it a beat longer say 5days or 6days? Would the active...
07 August 2018 3,028 3 View
When a liver organ is extracted from an experimental animal, they are quickly washed in ice cold 1.15% KCl solution.Why is it always KCl? Can't normal saline be used also?
04 May 2018 6,548 1 View
Can I store pulverized and completely dry leaf samples up to one year before extraction?
03 April 2018 6,490 4 View
I want to know how i can concentrate n-hexane plant extract and subsequently dry it so the shelf life can be extended.
31 December 2016 6,332 7 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?
29 July 2024 3,138 2 View
it's important to know that we are using the inactive elements of Cs, Sr, Co
25 July 2024 1,553 2 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.
17 July 2024 3,532 0 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View