Is there any possible explanation of a plant extract having a good in vitro antioxidant activity but poor in vivo protective activity or probably toxic in vivo? Any relevant article is highly appreciated.
Hi,
Even i did not got completely the question, but if you test it in vivo on an animal, for sure enzyme activity is an important issue (enzyme which metabolize the antioxidant). pH and temperature are the main factors for an enzyme activity, so they must be the same in both in vitro and in vivo test.
Dear please check the concentration invitro and invivo and also consider a particular physiological factors that will cause a maximize the invivo activity.
There are many possible reasons. First, I am assuming in vivo refers to some kind of animal model e.g. a mouse or rat.
if you administered the extract orally, one useful thing to check, if you know the exactly compound you are testing, would be the bioavailability of the compound in your invivo system. bioavailability is the amount of drug that is able to get into systemic circulation.
If you administered the compound via IV, bioavailability should not be a problem. However, the compound also has to be stable in the in vivo system too at body temperature. The compound might have been metabolized very quickly and thus lose its effect, or it might have acted at a site away from the target site.
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