I recently performed an RNA FISH experiment with five labels: Hoechst, GFP-tagged protein, Quasar570 RNA probe, mCherry reporter, and Quasar670 RNA probe. All probes are essential for the experiment. However, I'm seeking advice on image acquisition to minimize spectral bleed-through between Quasar570 and other fluorophores. Additionally, I'm open to suggestions for alternative fluorophores to replace Quasar570 that would exhibit minimal spectral overlap with the other labels.