Harsh Oza

8 Questions 10 Answers 0 Followers

Questions related from Harsh Oza

Can Quasar570 and mCherry fluorophores be imaged simultaneously/sequencially without significant spectral bleed-through or crosstalk?

I recently performed an RNA FISH experiment with five labels: Hoechst, GFP-tagged protein, Quasar570 RNA probe, mCherry reporter, and Quasar670 RNA probe. All probes are essential for the...

17 March 2024 9,760 0 View

Does actin binding probes like Spirochrome SPY650 FastAct effect the binding of other actin-binding proteins?

I am trying to measure the colocalization of actin and an actin-binding protein using live cell imaging. For labeling actin, I have used SPY650 FastAct which binds to the actin. Does this affect...

26 April 2023 3,646 0 View

What are the best methylation mimetic residues for arginine methylation?

I am exploring the role of Arginine methylation in the protein of my interest. I wanted to know which amino acid I could replace with arginine to mimic methylated arginine and non-methylated arginine.

27 September 2022 8,070 0 View

Can PFA fixation mislead localization of certain proteins in cells?

While visualising cells stained with antibodies, I see certain proteins getting localized inside the nucleus or other organelle. However, when I perform subcellular fractionation and probe for...

22 February 2022 1,562 3 View

How to measure actin-cortex width of the interphase cells? Any ImageJ plugin available?

I wanted to measure the width of the actin cortex on interphase cell images acquired through a confocal microscope. Can anyone provide a step-wise method to do so?

23 January 2022 2,051 4 View

Does small population of EGFP sticks or localizes on actin stress fibres or cortex? Does GFP antibodies interacts non-specifically in nucleus?

In one of my experiments, I used EGFP as control and I see some localization of EGFP on the actin cortex/stress fibres. Is this some non-specific binding of GFP antibody or some population of EGFP...

18 January 2022 8,052 2 View

Is there any ImageJ plugin which can help in automation of counting filopodia and membrane protrusion like tunneling nanotubes?

Tunneling nanotubes are long cytoplasmic bridges that connect two cells. These tube-like structures do not touch the surface (of coverslip) in vitro. I want to quantify these structures and...

22 December 2021 1,882 1 View

Is it normal to observe black dots (or precipitates) post plasmid transfection in mammalian cells (U2OS/HeLa)?

These dots are extremely small and do not move, however upon DAPI staining, I observe a lot of DAPI spots surrounding the nucleus and cells. Can it be mycoplasma contamination? I do not observe...

30 November 2021 6,163 5 View