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Questions related from Harsh Oza
I am exploring the role of Arginine methylation in the protein of my interest. I wanted to know which amino acid I could replace with arginine to mimic methylated arginine and non-methylated arginine.
27 September 2022 8,021 0 View
While visualising cells stained with antibodies, I see certain proteins getting localized inside the nucleus or other organelle. However, when I perform subcellular fractionation and probe for...
22 February 2022 1,558 3 View
In one of my experiments, I used EGFP as control and I see some localization of EGFP on the actin cortex/stress fibres. Is this some non-specific binding of GFP antibody or some population of EGFP...
18 January 2022 8,047 2 View
Tunneling nanotubes are long cytoplasmic bridges that connect two cells. These tube-like structures do not touch the surface (of coverslip) in vitro. I want to quantify these structures and...
22 December 2021 1,874 1 View
These dots are extremely small and do not move, however upon DAPI staining, I observe a lot of DAPI spots surrounding the nucleus and cells. Can it be mycoplasma contamination? I do not observe...
30 November 2021 6,157 5 View