Is there any ImageJ plugin which can help in automation of counting filopodia and membrane protrusion like tunneling nanotubes?

22 December 2021 1 2K Report

Tunneling nanotubes are long cytoplasmic bridges that connect two cells. These tube-like structures do not touch the surface (of coverslip) in vitro. I want to quantify these structures and currently do it manually using depth coding. However, it is time-consuming and has chances of errors.

Can anyone suggest if there is any way to automate the quantification of these tube-like structures?

Qamar Ul Islam

Harsh Oza

These articles might be helpful, take a look:

1. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5626553/

2. Article Correlative cryo-electron microscopy reveals the structure o...

3. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5612221/

4. https://rupress.org/jcb/article/220/5/e202101116/211896/Cytonemes-with-complex-geometries-and-composition

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