I am exploring the role of Arginine methylation in the protein of my interest. I wanted to know which amino acid I could replace with arginine to mimic methylated arginine and non-methylated arginine.
I recently performed an RNA FISH experiment with five labels: Hoechst, GFP-tagged protein, Quasar570 RNA probe, mCherry reporter, and Quasar670 RNA probe. All probes are essential for the...
16 March 2024 9,789 0 View
I am trying to measure the colocalization of actin and an actin-binding protein using live cell imaging. For labeling actin, I have used SPY650 FastAct which binds to the actin. Does this affect...
25 April 2023 3,667 0 View
While visualising cells stained with antibodies, I see certain proteins getting localized inside the nucleus or other organelle. However, when I perform subcellular fractionation and probe for...
21 February 2022 1,573 3 View
I wanted to measure the width of the actin cortex on interphase cell images acquired through a confocal microscope. Can anyone provide a step-wise method to do so?
22 January 2022 2,071 4 View
In one of my experiments, I used EGFP as control and I see some localization of EGFP on the actin cortex/stress fibres. Is this some non-specific binding of GFP antibody or some population of EGFP...
17 January 2022 8,065 2 View
Tunneling nanotubes are long cytoplasmic bridges that connect two cells. These tube-like structures do not touch the surface (of coverslip) in vitro. I want to quantify these structures and...
21 December 2021 1,907 1 View
These dots are extremely small and do not move, however upon DAPI staining, I observe a lot of DAPI spots surrounding the nucleus and cells. Can it be mycoplasma contamination? I do not observe...
29 November 2021 6,178 5 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...
05 August 2024 3,246 1 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
When we conduct linear regression, there are several assumptions. The assumption of normality is whether the residual errors are normally distributed, not whether a predictor is normal?
31 July 2024 6,163 3 View
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
31 July 2024 2,030 1 View
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View
I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...
28 July 2024 3,559 0 View
I wanted to know whether we can observe the synergistic/antagonistic/additive properties of combinations or mixtures of compounds through docking analysis. But during docking preparation any...
28 July 2024 7,413 6 View
I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...
23 July 2024 3,891 0 View
Kao's panel cointegration tests , is there anyone willing to explain me the eviews-9 output for the Kao's panel cointegration tests?
23 July 2024 5,051 4 View