Can mycoplasma contamination happen without the spakes shape appear under microscope?

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Why the clear zone indicating amylase activity on agar plates dissappears after some hours?

I did qualitative assay on agar plates with starch as sole carbon source for checking amylase activity, once I added iodine the clear zone appeared arround the colonies but after some hours and...

13 May 2024 6,529 2 View

My recombinant enzyme activity is acting weird, does anyone have an explanation, or even know how to solve this problem?

I'm working with recombinant protein, it's a protease enzyme that we use E. coli to synthesize, and usually, it's stable for up to six months in the -80 freezer. Recently, the protein activity has...

17 April 2024 4,262 3 View

Sub-cloning not working?

I am a graduate student doing sub cloning of two similar genes into pBC SK(+); one of the gene was inserted but few colonies appeared on the agar after transformation, the other is not inserting...

22 February 2024 7,237 5 View

How can audiologists enhance patient-centered care in their practice?

In light of recent research findings and advancements in communication strategies for individuals with hearing loss, how can audiologists enhance patient-centered care in their practice? Are there...

17 December 2023 2,894 1 View

Why does performance point still in elastic range ?

I have a four-story steel structure When conducting a pushover analysis and examining the results, the structure's ductility ratio at the performance point did not exceed 1, and the equivalent...

08 November 2023 934 4 View

Cadmium sulfide nanoparticles from banana extract , what is the problem?

I'm preparing Cadmium sulfide nanoparticles using banana extract as a capping agent, after drying it, it looks like thin sheets, cannot be grinded as powder (The last time, it does not look like...

12 June 2023 3,604 1 View

Safe cities for women with disabilities ?

Safe cities for women with disabilities

13 May 2023 8,560 3 View

Can I use QIAamp DNA Stool Mini Kit for DNA extraction from a bacterial colony? if anyone tried this and what are the necessary protocol adjustments?

#DNA_extraction #QIAamp #Qiagen #Colony_DNA

04 May 2023 4,548 2 View

How to solve the problem if the sensor is not capable of transmitting data to base station in WSN. Is there any paper talking about this issue?

25 January 2023 5,399 4 View

Why enzymes couldnot migrate from wells to stacking gel in electrophoresis? Is there is problem in its precipitation?

i have precipitated enzyme by acetone.. when i use coomassie staining in native gel electrophoresis, it couldnot migrate to stacking and then resolving gel. is there is a problem in acetone...

10 January 2023 2,353 5 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

May members post flyers about opportunities to present at a conference? If so, where to post?

May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/

08 August 2024 4,585 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Hello all, Looking for international reviewer to review Ph.D thesis in wireless sensor network.Can anybody help?

My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...

07 August 2024 4,600 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View