If scientists got some money, what they will do with it?

If scientists got some money, what they will do with it?

07 March 2018 2,235 13 View

Can MetaPhor agarose compete with polyacrylamide?

I have a procedure involves the using of polyacrylamide, but I don't have it! Can I use MetaPhor instead? What limits the using of MetaPhor instead of polyacrylamide?

01 March 2018 2,866 2 View

What kind of relationship between DNA methylation level and gene expression?

I investigated the relationship between DNA methylation level and gene expression in different plant species under different affects, and I found it negative in general. I get confused when I read...

01 March 2018 4,043 4 View

Why does plant still activating the mechanisms of stress tolerance even if growth factors are adequately secured?

The plant in the attached image is a wild type! It have been grown in pots and all growth conditions were in educate levels. Why does the plant still show symptoms of stress! What is the role of...

26 February 2018 1,677 6 View

What is the suitable kit for extracting DNA from seed tissue?

In many occasions I have problems with DNA extraction from seeds tissue, especially wild types seeds.I tried different protocols, but i still have invalid DNA! Did any body experienced an...

23 February 2018 8,183 4 View

How to evaluate the results of the DNA sequencing?

When you receive DNA sequencing results as in the attached image, is it generally possible to say that the results of the upper row are more confident than the results of the lower in the same image?

28 January 2018 1,229 2 View

Which of the SMAP Passive Soil Moisture products are the best one?

The NASA Soil Moisture Active Passive (SMAP) Produce Soil Moisture Product in HH, VV and dual polarization. Which of these products are the best one? Which of these products are suitable in...

20 July 2017 4,860 3 View

How can I convert Satellite Soil Moisture data (m3/m3) into mm?

30 May 2017 1,064 7 View

Is ther anyone use Preston tube to measure bed shear stress in open channel?

15 January 2017 9,938 5 View

How we can calculate soil surface roughness without field measurement?

Mapping surface soil moisture from the SAR images is a demonstrated procedure, but several factors can interfere with the interpretation and must be taken into account. The most important factors...

15 July 2016 3,747 5 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Open Source IoT Simulators supported by Machine Learning?

Dear Research Gaters, Let me first add context to the question. I am looking to simulate the waste management system and resources of a city. The hardware devices I need to simulate include,...

21 February 2021 2,333 4 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

How to correctly choose the number of solution intervals when preforming harmonic analysis in ANSYS?

Good day, I design the rail-wheel geometry and the harmonic frequency ranges between 20-10kHz with 500Hz solution intervals and the results makes sense. Most of the time on the related literatures...

19 February 2021 3,190 3 View