Can lipofectamine be used for generation of large gene(6-7k bp) knock-in thru CRISPR mediated HDR?

14 September 2022 1 5K Report

This was my original plan, however I was just reading this review Article A review of emerging physical transfection methods for CRISP...

and it states that "When using lipofection, the cargo only reaches the cytoplasm, which is unfortunate for cargo such as plasmid DNA which needs to reach the nucleus to function". This is an unsourced claim, and I have read many people on here claiming to use lipofectamine for generation of stable lines, as well as studies that use ssODN's to make small edits/conversions thru HDR. However I have not been able to find a study that uses CRISPR mediated HDR for stable integration of large genes. If anyone could point me in the right direction I would appreciate it.

Di Xia

That doesn't make sense. If the lipofection can not delivery plasmid into the nucleus, how can a GFP plasmid transfected by lipofectamine to be expressed through transcription, which happens in the nucleus?

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