How necessary is it to have a G after the start codon for efficient translation?

06 February 2023 0 7K Report

I have been under the impression a Kozak is just GCCACC preceding the start codon, but just learned it also includes a G just after the start codon, i.e. GCCACC ATG G. If I am encoding a peptide that does not start with a G just after the start codon, will it still translate efficiently? The peptide has a threonine after the methionine so I can't just change it to a codon that starts with a G.

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