Hi,

It sounds like you're interested in measuring a variety of markers related to aging in blood samples from your participants. The specific lab or institution that can perform these tests will depend on the location and resources available to you.

In general, many research institutions and commercial labs offer services for measuring biomarkers in blood samples. Some examples include university core facilities, hospitals, and private research and diagnostic labs. You might want to look into the availability of these services within your academic institution, or by contacting commercial laboratories.

It's important to note that some markers (especially the proteins) might need special preservation of the samples, like freezing the plasma/serum samples at -80C and avoiding any thawing before analysis, so make sure that this aspect is covered when looking for the lab.

With regard to the volume of blood you'll need, it can vary depending on the specific markers you're measuring. For protein markers, most protocols will require around 100ul of plasma or serum. For markers that require genomic analysis like DNA assays, the volume required will typically be higher, around 1-2ml of blood.

As for the specific markers you listed:

• Resolvins/Protectins (D1,D2,D3) are lipid mediators that can be measured in plasma by ELISA or mass-spectrometry-based methods.
• Klotho proteins can be measured in plasma by ELISA or mass-spectrometry-based methods.
• Sirtuins can be measured in plasma by ELISA or mass-spectrometry-based methods.
• Neurofilament light chain protein can be measured in plasma by ELISA or mass-spectrometry-based methods
• Glial Fibrillary acidic protein can be measured in plasma by ELISA or mass-spectrometry-based methods
• Tau Protein can be measured in plasma by ELISA or mass-spectrometry-based methods
• Alarmins can be measured in plasma by ELISA or mass-spectrometry-based methods
• Circulating senescent cells can be measured by flow cytometry, after isolation and staining of specific markers like p16INK4a, p21, etc.

It's recommended to check the literature and consult with the lab you will use to confirm the specific volume and preservation needed for your markers, and to optimize the assay conditions.

Regards.