The buffer contains: 100mM NaCI, 0.1% Triton X-100, 300mM Sucrose, 1mM MgC2, 1mM EGTA, 10mM PIPES (pH 6.8), and 100uM PMSF
Adam B Shapiro
I would not use it. First, the PMSF is no longer intact. This compound rapidly degrades in water. You could add fresh PMSF to replenish it, but second, the Triton X-100 will have oxidized and could even be destructive due to reactive oxygen species.
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