What could be the reason for the band of (amyloid fibril) not appearing in the gel?

I am studying the glycation mediated amyloid fibril formation. When there is native protein + fibrils i am able to see the bands in Tris-Tricine SDS PAGE. But, when i precipitate the fibrils, by...

05 June 2019 3,389 5 View

Can anyone suggest me the easy way to change the amino acid residue to an adduct (For eg. Arginine to argpyrimidine) in the pdb file.

I like to replace arginine with argpyrimidine. It is easy to change the amino acid, but i am not finding a way how to add a new chemical structure in the place of an existing amino acid. Thanks...

04 May 2017 8,988 3 View

Can anyone help me with a technique to isolate alkaloids from spinach?

I am trying to isolate alkaloids from spinach. I am having methanol extract. I am planning to do 5% citric acid-ethyl acetate partition followed by addition of ammonia and extraction with ethyl...

06 July 2014 6,333 13 View

Can you suggest a method for purification and crystallization of leaf extract?

06 July 2014 3,682 23 View

No title

Separation of salts from phytochemicals.

06 July 2014 2,657 4 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

How to calculate Total Flavonoid?

I analyzed flavonoid total spectrophotometry, the procedure as below: 2 ml supernatan added 5 ml dd.H2O, then added 0,15 ml NaNO2 5%, 5 minutes later added 0,15 ml AlCl3 10%, After 6 minutes,...

01 March 2021 6,722 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View