Can I use Sephacryl-100 column for flavonoid purification?

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What could be the reason for the band of (amyloid fibril) not appearing in the gel?

I am studying the glycation mediated amyloid fibril formation. When there is native protein + fibrils i am able to see the bands in Tris-Tricine SDS PAGE. But, when i precipitate the fibrils, by...

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Can anyone suggest me the easy way to change the amino acid residue to an adduct (For eg. Arginine to argpyrimidine) in the pdb file.

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Can anyone help me with a technique to isolate alkaloids from spinach?

I am trying to isolate alkaloids from spinach. I am having methanol extract. I am planning to do 5% citric acid-ethyl acetate partition followed by addition of ammonia and extraction with ethyl...

06 July 2014 6,456 13 View

Can you suggest a method for purification and crystallization of leaf extract?

The extract was obtained using silica gel column purification. The fraction was collected with methanol. It was showing a streak in TLC. I wish to crystallize a purified fraction from plant...

06 July 2014 3,808 23 View

I am getting salt impurities with my phytochemical fractions? Can anyone help with the ways to eliminate it?

Separation of salts from phytochemicals.

06 July 2014 2,751 4 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

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Is there a problem with my RNA pellet?

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Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

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GC-MS retention index prediticon?

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RNA Extraction Using Hot Borate Method No Longer Working?

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I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

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Does crude extraction using NaOH and Tris work well with Fungi?

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Can I use a HisTRAP column for affinity chromatography?

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Separation of organic acids-HPLC?

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07 August 2024 8,782 3 View

Rajesh K P

Its better to use sephadex LH-20... Before going to sephadex, run your sample through amberlite XAD and then get elute the adsorbed phenolic compounds from amberlite using methanol. Dry the elute and run that sample in sephadex LH-20.

Ravi Kant Upadhyay

You can use reverse phase column using 25um-50um size beads. Simple columns are more preferable to isolate flavonoids