Govindarajan Prasanna

6 Questions 22 Answers 0 Followers

Questions related from Govindarajan Prasanna

What could be the reason for the band of (amyloid fibril) not appearing in the gel?

I am studying the glycation mediated amyloid fibril formation. When there is native protein + fibrils i am able to see the bands in Tris-Tricine SDS PAGE. But, when i precipitate the fibrils, by...

06 June 2019 3,460 5 View

Can anyone suggest me the easy way to change the amino acid residue to an adduct (For eg. Arginine to argpyrimidine) in the pdb file.

I like to replace arginine with argpyrimidine. It is easy to change the amino acid, but i am not finding a way how to add a new chemical structure in the place of an existing amino acid. Thanks...

05 May 2017 9,051 3 View

Can you suggest a method for purification and crystallization of leaf extract?

The extract was obtained using silica gel column purification. The fraction was collected with methanol. It was showing a streak in TLC. I wish to crystallize a purified fraction from plant...

07 July 2014 3,773 23 View

Can anyone help me with a technique to isolate alkaloids from spinach?

I am trying to isolate alkaloids from spinach. I am having methanol extract. I am planning to do 5% citric acid-ethyl acetate partition followed by addition of ammonia and extraction with ethyl...

07 July 2014 6,420 13 View

I am getting salt impurities with my phytochemical fractions? Can anyone help with the ways to eliminate it?

Separation of salts from phytochemicals.

07 July 2014 2,718 4 View

Can I use Sephacryl-100 column for flavonoid purification?

I am purifying flavonoids from leaf extract of a plant. I wish to do HPLC for purification. Can I use sephacryl columns 100, 200 (GE) for purification?

07 July 2014 2,763 2 View