Can I use Phosphate Buffer for Antibody and Fluorescence conjugation?

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How to remove the salt and get purified DNA in Silica Magnetic bead-based Nucleic acid extraction?

I am doing nucleic acid extraction (manual silica magnetic bead-based) from the serum sample. After the extraction, I have taken the OD 260/280 in UV spec. There is no protein contamination. But...

04 November 2020 1,061 2 View

Could you tell me the composition of the wash buffer in RNA extraction (Silica Magnetic Bead-based) and its proportions?

I am doing silica magnetic beads-based RNA isolation. I am facing some impurity issues because of washing. So kindly share some best washing buffer composition of wash buffer for especially for...

01 November 2020 6,536 2 View

Best book for Fluid Structure Interaction?

Practical methods along with the basic behind Fluid Structure Interaction.

11 June 2020 9,743 9 View

Most of the Research papers are found more theoretical and previous work but only few paragraph spent in experiment and result , Why ?

My research is going on Traffic analysis , Prediction and Intelligent Transport System. I have good concept in theory but I found that most of the papers are not elaborated experiments setup...

17 April 2020 407 12 View

Do I need to do a polymerization reaction under a nitrogen atmosphere?

I am polymerizing styrene with Potassium Peroxodi Sulfate and Acrylic acid by an emulsion polymerization method. The literature I am referring to mentions stirring the reagents with a nitrogen...

18 December 2019 8,158 4 View

IoT Challenges with Healthcare?

In want do some work in the security of IoT with Healthcare , please suggest me some area , in which area our scholar can do some work. If possible provide some detail from 2018 and 2019 Dr....

12 July 2019 6,628 3 View

Performance Analysis of Big Data ?

How performance of the Big data can be analysis, which framework should used ? Is Hadoop and Spark Framework are good or other tool should use , is any Source for code or algorithm can find?

01 July 2019 348 3 View

I want to study about antibody antigen binding for chikunguniya and malaria virus? Where can i find relevant material?

Study for Virus diagonesis

23 April 2019 9,210 5 View

Address of Tanusri Mandal is wrong . How to rectify?

Tanusrei Mandal, an author of the paper, entitled "Calcite solubilization by Bacteria: An Novel method of Environmental pollution control ". Published in Geomicrobiology Journal Vol.32, PP.846...

23 January 2018 1,566 3 View

Bandgap Engineering of BTO/bottom electrode/STO (or any suitable substrate) for Photovoltaic Applications.

I am interested in reading some literatures on the Bandgap Engineering of BTO/bottom electrode/GSO (or any substrate), could someone provide me some links of interesting papers on the same?

06 August 2017 3,989 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View