Amal Senevirathne

8 Questions 40 Answers 0 Followers

Questions related from Amal Senevirathne

Can anyone provide me a python code to add column names to a very large dataframe?

Dear all, I have a data frame with a very large number of columns (let's say 90,000 columns) and I want to add a column ID for each column. I know how to do it in R. But couldn't figure out the...

19 April 2023 8,909 2 View

How to extract lowest binding energy from thousand Vina dock log files and put in a txt file using python?

Dear All, I have executed >1000 drug molecules in a Vina docking study. Now I have >1000 log files that contain the top ten binding scores for every compound in a log file for each. I want to...

07 November 2022 547 3 View

How to get a price quotation for ZINC15 compounds?

I want to purchase some drug compounds from ZINC15 database. I wonder how to get a quotation to estimate the cost of selected compounds. Thank you, Amal.

03 February 2022 266 4 View

Why some viral proteins are unable to express and purify in pET28a expression and purification system?

Dear All, I am trying to clone and purify codon-optimized viral proteins using pET28a expression system. Even though I have the perfect insert in the recombinant plasmid, there was absolutely no...

25 September 2020 3,770 8 View

Is it possible to use Ni-NTA resin for pull-down assay using his tag protein?

Dear all, Is it possible to use Ni-NTA resin for pull-down assay using his tag protein?. Will it get a high background effect? Thank you, Amal.

03 June 2020 6,874 7 View

Dear all, Can anybody tell me where I can purchase pCP20 plasmid that is used for lambda red recombination experiments?

Dear all, I have to find pCP20 plasmid system to be used in in-frame gene deletion experiment. Can anyone tell me, whether it is commercially available to purchase?. Thank you, Amal.

29 January 2018 8,023 2 View

Does anybody knows how to use prokka annotation pipeline for annotating prokaryotic genomes?

I have a bundle of sequence data need to be annotated. I want to try prokka annotation pipeline for this activity. (http://www.vicbioinformatics.com/software.prokka.shtml), but I have no idea how...

18 March 2017 2,472 6 View

What would be the ideal medium for hybridoma cells during cloning by limiting dilution?

There are many different medium combinations for hybridoma expansion. I have conducted fusion of Sp2/0 Ag 14 cell line with B cells from balb/c mice. I am using DMEM-20/ 10 mM HEPES/1 mM sodium...

29 April 2014 2,835 3 View