15 Questions 54 Answers 0 Followers
Questions related from Amal Senevirathne
Dear all, I have a data frame with a very large number of columns (let's say 90,000 columns) and I want to add a column ID for each column. I know how to do it in R. But couldn't figure out the...
19 April 2023 8,995 2 View
Hi, For a simulated genomic analysis, I am trying to use QMSim software. Can anybody tell me how I can convert QMSim-generated output files that are in *.txt format, to plink input format? If you...
21 December 2022 6,689 0 View
Dear All, I have executed >1000 drug molecules in a Vina docking study. Now I have >1000 log files that contain the top ten binding scores for every compound in a log file for each. I want to...
07 November 2022 660 3 View
I want to purchase some drug compounds from ZINC15 database. I wonder how to get a quotation to estimate the cost of selected compounds. Thank you, Amal.
03 February 2022 332 4 View
Dear all, Is proteinase K a glycosylated protein? Is it possible to produce proteinase K or proteinase K like proteins in bacterial systems? Will the activity be preserved for bacterial origin...
29 October 2021 5,124 9 View
Dear all, Acriflavine agglutination test is being used as a common biochemical test to differentiate smooth and rough strains of bacteria such as Brucella spp and Salmonella spp. What is the mode...
27 August 2021 6,011 2 View
Dear All, I am trying to clone and purify codon-optimized viral proteins using pET28a expression system. Even though I have the perfect insert in the recombinant plasmid, there was absolutely no...
25 September 2020 3,900 8 View
SLURP1 protein is known to be an anti-cancer protein. It can inhibit certain cancer cell lines at very low concentrations as observed in in vitro experiments. However, gene expression data on NCBI...
01 September 2020 7,236 5 View
Dear all, Is it possible to use Ni-NTA resin for pull-down assay using his tag protein?. Will it get a high background effect? Thank you, Amal.
03 June 2020 6,938 7 View
Dear Friends, I am trying to make SifA deleted Salmonella Typhimurium strain using lambda red recombination procedure. I was following standard procedure using pKD46, pKD3 and pCP20 plasmids....
24 July 2018 193 2 View
Dear all, I am trying to amplify mice IL-15 cytokine. I was trying RNA extratced from mice splenocytes, kideny cells both with and without stimulation for cDNA synthesis. However, still I did not...
12 May 2018 2,384 2 View
Dear all, I have to find pCP20 plasmid system to be used in in-frame gene deletion experiment. Can anyone tell me, whether it is commercially available to purchase?. Thank you, Amal.
29 January 2018 8,099 2 View
I have a bundle of sequence data need to be annotated. I want to try prokka annotation pipeline for this activity. (http://www.vicbioinformatics.com/software.prokka.shtml), but I have no idea how...
18 March 2017 2,534 6 View
We are trying to develop a bio sensing strip using monoclonal antibodies to detect bacteria. In this we have immobilized a monoclonal antibody (Capturing antibody) on a membrane and the same...
02 July 2015 6,731 5 View
There are many different medium combinations for hybridoma expansion. I have conducted fusion of Sp2/0 Ag 14 cell line with B cells from balb/c mice. I am using DMEM-20/ 10 mM HEPES/1 mM sodium...
29 April 2014 2,897 3 View
