We found an old dry packaged dialysis tubing in the lab and plan to use them for buffer exchange prior to ion exchange chromatography. It was kept in room temperature with no visible sign of damage. However, the tubes were old as they were purchased in 1993. and were estimated to expire in the year 2000. Can I still use them as I was looking for another method to conduct buffer exchange other than via concentrator and desalting resin.
The potential problem with old dialysis tubing is that it becomes dried out and brittle and has cracks in it. So you could possible lose your sample. But if it looks good, I would test it to be sure it holds liquid and doesn't leak before risking your sample.
Test it first. Add some BSA (or other protein that you don't mind risking) to the tubing in an isotonic solution and see if the concentration changes over time.
Thank you Ryan Wheeler Michael J. Benedik for the answers! I will test the tubes first!
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