MN9D cell line is a fusion of rostral mesencephalon of E14 mice and neuroblastoma cell.
Thanks,
Manal.
Hi Manal - the brief answer is yes, you should be able to use mouse antibodies as this line was generated from mice. If you are unsure however try to find antibodies which cross react across species (for example this LMX1b antibody is pan-species: http://www.abcam.com/lmx1b-antibody-ab129306.html)
You should also check that the antibody is capable of IHC (the example above is useable for WB and IHC-P). Also once you have chosen the IHC antibody check on Google if it has been used in any publications for IHC (as this will give the AR conditions etc. and also give you an idea of the image quality to expect).
With regards to MN9D cells themselves there has been some doubts raised that they do truly represent dopaminergic neurons: http://www.ncbi.nlm.nih.gov/pubmed/17028430
I hope this helps,
Gary
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