Trouble shooting with his tagged protein expression in E.Coli?

i have issues in expressing a chicken protein in BL21 cells with his tag. I do not see induction even at 18 degrees. Any suggestions

15 February 2021 8,939 3 View

Two different gaussian width in QM/MM Metadynamics?

Is it wise to use two different gaussians for two CVs during QM/MM metadynamics simulations?. Example for 0.5kcal/mol Gaussian height, For CV1 width is 0.3 and CV2 is 0.15. Where both CVs address...

10 March 2020 5,287 3 View

What is the impact of correlation test in Image encryption ?

Is correlation test sufficient for proving the efficiency of the encryption scheme?

23 February 2020 3,524 4 View

What is the significance of image entropy ( plain image and cipher image) in image processing?

Whether increase or decrease in entropy of cipher image is good for encryption? How?Why?

06 May 2019 9,299 12 View

Can PCR master mix solution be prepared in advance and stored at -20C?

For doing PCR, instead of preparing the PCR mater mix solution each and every time, is it advisable to prepare it in a larger quantity and store it at -20C so that the PCR can be set up faster....

27 January 2019 7,112 19 View

What are the other options to play with audio files apart from shifting and transformation?

Any other specific operations with speech files?

23 September 2018 9,243 2 View

Can I stain cells with Annexin V after fixing with 70% ethanol?

I have treated cancer cells with inhibitors of cell cycle. In addition to looking at the stage of cell cycle they are in, I want to see if there is any apoptosis induced by the treatment. But I...

28 July 2018 1,242 3 View

How to measure the mesh size in COMSOL for PCF design for FEM analysis?

I want to know how to measure mesh size in COMSOL when are using initializing mesh than refined mode? Is that possible?

28 February 2018 4,418 3 View

Analytical formula of Natural Freqency of a composite with fixed-free (cantilever type) boundary condition?

I need to calculate the analytical relation for frequency of a cantilever composite beam, where one beam is prestressed on which an another non-prestressed beam is placed in a way to form the...

17 January 2018 2,773 9 View

What are the reasons for getting low value of R-square?

In my research I am getting a low value of R-square (0.253). What are the possible reasons for getting low value and How it can be interpreted?

16 September 2017 2,697 3 View

Which polarizers are usually used to measure the birefringence of zebrafish muscles?

I already found several publications on that topic, however, the specifications for the used filters are often missing. Some more specific information than "fish is placed between to polarizing...

23 February 2021 1,934 1 View

How to calculate the Atomic Polar Tensor using DFT ?

Dear friends, I am a beginner at DFT calculation. I have done a structure optimization using DFT- B3LYP/6-31G*, Nevertheless, I don't know How to calculate the Atomic Polar Tensor (APT) in...

17 February 2021 9,082 3 View

Do you have any experience with transfection of EBV immortalized B cells with Lipofectamine?

I know that efficiency of transfection for primary B cells is very low with Lipofectamine. But I did not try that for EBV immortalized B cells. Do you have any experience with transfection of EBV...

05 February 2021 4,159 1 View

Should I make 2 helical antennas? One for 2.4 GHz and another for 5.8 GHz?

Should I make 2 antennas, one for 2.4 GHz and one for 5.8 GHz? How should I approach the problem if I want to design the antennas which cover 400 and 900 MHz and 2.4 and 5.8 GHz? All together in...

26 January 2021 906 3 View

What is the best concentration of Pam3CSK4 to coat a dish to activate with THP-1?

Hi everyone I am trying to coat a glass-bottom dish with Pam3CSK4 and plate THP-1 cells to measure IL6 with ELISA from the supernatant. I wondered if anyone knows a good concentration and...

21 January 2021 2,593 1 View

SPSS - PLUM: Shall I introduce binary variables as covariates?

I am doing an analysis of interaction effects, using PLUM in SPSS. My question is if the binary variables, that i have constructed to analyze the interaction effects, should be introduced as...

17 January 2021 8,217 4 View

Can you polarize Jurkat cells into different T helper subsets?

I am wondering if anyone has had success with in vitro polarization (via cytokines) of Jurkat cells. My goal is to understand the affect of a drug on Treg induction and differentiation. I...

12 January 2021 3,606 2 View

What is the effect of polarization of unit elements in an IRS, if its built for mmWave application?

I usually don't see path loss models for IRS consider polarization. But specific to mmWave application, the polarization of the antenna has some effect. Is there any research that has explored this?

08 January 2021 3,054 1 View

Guidance in choosing a column for UHPLC quantification of organic acids in cranberry and blueberry fruit?

07 January 2021 6,214 3 View

How aromatics can dissolve asphaltene ?

As we all know asphaltene is defined as " The highest molecular weight component in the crude oil that is insoluble in light n-alkanes such as n-pentane or n-heptane and soluble in aromatics such...

05 January 2021 6,822 2 View