Alexandra Johnson

2 Questions 152 Answers 0 Followers

Questions related from Alexandra Johnson

Is it possible to elute a 14x his tagged protein off of a cobalt column with imidazole?

I have a construct that goes (N to C terminus) 14x His tag - SUMOstar cleavage tag - Protein of Interest (POI). The initial idea was to elute using on-column cleavage of fusion protein, but this...

23 June 2021 4,684 10 View

Does cleaning up cDNA after reverse transcription reduce late cycle background?

I've been wondering if removing the random primers from the reverse transcription reaction either with a column or precipitation before using it as a qPCR template reduces the background a bit in...

26 December 2020 6,409 4 View