When will we have access to Brazilian agricultural censuses microdata publically available? Can Google and Microsoft help it?

Microdata are a wonderful way of analyzing specific issues in an heterogeneous environment. Brazil is wonderfully diverse country, and its countryside is also extremely heterogeneous. We have...

25 October 2020 8,900 2 View

Northern Blot or PCR?

Hello! I intended to measure the differential expression of three proteins of the same family before and after a stimulus, but i don't know which is the most suitable or reliable method to do so....

16 May 2020 6,562 3 View

Positive Zeta Potential NPs dispersed in water?

Hello guys I am looking for positive zeta potential colloidal particles dispersed in water which I can order online. pH around 6 to 7. The size its from 300 nm until 700 nm. Thanks a lot for...

28 March 2020 6,869 3 View

What hands-on approaches to teach Computer Assisted Qualitative Data Analysis (CAQDAS)?

13 December 2017 2,060 8 View

What do you think about the statement "Do not use a preservative solution in soil solution collectors (lysimeters) left in the field for a week"?

The concentration of the preservative solution can be very high if soil solution volume collected is very low. In the case of a strong acid solution, it can affect biogeochemical processes in the...

03 July 2014 9,957 3 View

Blood Perfusion during thermal stress

How does blood perfusion change during hyperthermia and hypothermia? What mathematical expressions exist for these phenomena? I would like indications of research about the subject. Thank you!

01 January 1970 6,395 2 View

Why does the SCell need to be time-synchronized to the PCell in LTE-LAA deployments?

My question regards the real necessity to have subframe (3GPP Rel. 12) or slot (3GPP Rel. 13) aligned transmissions in the unlicensed band. According to TR 36.889: "Channel reservation refers to...

01 January 1970 7,746 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View