When will we have access to Brazilian agricultural censuses microdata publically available? Can Google and Microsoft help it?

Microdata are a wonderful way of analyzing specific issues in an heterogeneous environment. Brazil is wonderfully diverse country, and its countryside is also extremely heterogeneous. We have...

25 October 2020 8,900 2 View

Northern Blot or PCR?

Hello! I intended to measure the differential expression of three proteins of the same family before and after a stimulus, but i don't know which is the most suitable or reliable method to do so....

16 May 2020 6,562 3 View

Positive Zeta Potential NPs dispersed in water?

Hello guys I am looking for positive zeta potential colloidal particles dispersed in water which I can order online. pH around 6 to 7. The size its from 300 nm until 700 nm. Thanks a lot for...

28 March 2020 6,869 3 View

What hands-on approaches to teach Computer Assisted Qualitative Data Analysis (CAQDAS)?

13 December 2017 2,060 8 View

What do you think about the statement "Do not use a preservative solution in soil solution collectors (lysimeters) left in the field for a week"?

The concentration of the preservative solution can be very high if soil solution volume collected is very low. In the case of a strong acid solution, it can affect biogeochemical processes in the...

03 July 2014 9,957 3 View

Blood Perfusion during thermal stress

How does blood perfusion change during hyperthermia and hypothermia? What mathematical expressions exist for these phenomena? I would like indications of research about the subject. Thank you!

01 January 1970 6,395 2 View

Why does the SCell need to be time-synchronized to the PCell in LTE-LAA deployments?

My question regards the real necessity to have subframe (3GPP Rel. 12) or slot (3GPP Rel. 13) aligned transmissions in the unlicensed band. According to TR 36.889: "Channel reservation refers to...

01 January 1970 7,746 4 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Good sites to recruit survey participants on a grad school student budget?

Any good recommendations for participant recruitment for online surveys on a grad school student budget?

02 March 2021 4,293 4 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View