9 Questions 3 Answers 0 Followers
Questions related from Wilsea Maria Figueiredo
A need to analyse specific sequences gathered from genbank in order to analyse primers matching/designing. I have used Sequencher (gene codes) in the past and I loved it. Yet prices are now...
25 April 2024 239 3 View
Having problems with dimers and an answer from Paul Rutland for another question made me think that the fact that I store my PCR mix in the fridge might be the problem. I must add that in this mix...
29 March 2024 9,748 3 View
I have so far disliked Taqs that come with dyes because dye concentration seems excessive to me. I have recently bought a Taq with dye-free buffer, I wonder if I can use a small bit of gel loading...
21 March 2024 3,170 0 View
Hi all. I have some primer pairs which always produce those horrible primer dimer bright smear! Increasing annealing temperature does not solve the problem. Any suggestiopn for a PCR enhancer or...
20 March 2024 6,026 4 View
Need do look for milk bacterial pathogens using PCR. Does anyone suggest me a good protocol for DNA extraction? I have heard milk proteins are difficult to deal with aabd act as taq inhibitors
14 March 2024 354 0 View
I am making tests for diagnostic PCRs. At the moment I am trying to standardize a pcr reaction for a circa 180 bp product. I did not get positive bands so far. Interestingly, I get what appears to...
27 February 2024 2,766 3 View
We would like to preserve RNA from mammal blood samples. Unfortunately the only product I am aware is RNA Later and it is very very expensive for our limited budget. Any ideas? Thank you very much
13 December 2023 6,130 2 View
Sorry basic question. I am aware primers should be described in the 5'-3' direction in the literature. When I am ordering primers I have taken from published papers, should I use the same sequence...
19 October 2023 5,494 3 View
We are currently having problems with PCR amplifations of a ~200bp gene fragment. We cannot see any product after a 35 cycles amplification round and usually use a simple reamplification protocol:...
28 September 2023 9,182 1 View
