Need do look for milk bacterial pathogens using PCR. Does anyone suggest me a good protocol for DNA extraction? I have heard milk proteins are difficult to deal with aabd act as taq inhibitors
A need to analyse specific sequences gathered from genbank in order to analyse primers matching/designing. I have used Sequencher (gene codes) in the past and I loved it. Yet prices are now...
24 April 2024 259 3 View
Having problems with dimers and an answer from Paul Rutland for another question made me think that the fact that I store my PCR mix in the fridge might be the problem. I must add that in this mix...
28 March 2024 9,886 3 View
I have a dataset of trace elements LA-ICP-MS analyses with raw data, which consists of a set of a single file for each spot analysis. Each of these files has some columns (masses) and plenty of...
24 March 2024 898 6 View
I have so far disliked Taqs that come with dyes because dye concentration seems excessive to me. I have recently bought a Taq with dye-free buffer, I wonder if I can use a small bit of gel loading...
20 March 2024 3,193 0 View
Hi all. I have some primer pairs which always produce those horrible primer dimer bright smear! Increasing annealing temperature does not solve the problem. Any suggestiopn for a PCR enhancer or...
19 March 2024 6,067 4 View
I am making tests for diagnostic PCRs. At the moment I am trying to standardize a pcr reaction for a circa 180 bp product. I did not get positive bands so far. Interestingly, I get what appears to...
26 February 2024 2,820 3 View
We would like to preserve RNA from mammal blood samples. Unfortunately the only product I am aware is RNA Later and it is very very expensive for our limited budget. Any ideas? Thank you very much
12 December 2023 6,163 2 View
Sorry basic question. I am aware primers should be described in the 5'-3' direction in the literature. When I am ordering primers I have taken from published papers, should I use the same sequence...
18 October 2023 5,523 3 View
We are currently having problems with PCR amplifations of a ~200bp gene fragment. We cannot see any product after a 35 cycles amplification round and usually use a simple reamplification protocol:...
27 September 2023 9,212 1 View
Its manual doesn't have the conversion factor as Brookfield's does. I wish to convert rpm to shear rate but I don't know how.
08 April 2023 4,351 1 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hello everyone, I am researching ways to enhance the quality of lamb milk replacer for machine feeding. I would appreciate any insights or recommendations on the following: Manual vs. Machine...
06 August 2024 6,227 2 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26
04 August 2024 9,919 4 View
How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?
02 August 2024 9,644 0 View