Hi, I want to obtain circular RNA and lariat. I need treat my samples with DNase I and RNase R. Do you know, I can do this in the same time? And maybe do you have any protocols for simultaneous treatment with DNase I and RNase R?
I used the Intact Genomics FastAmp® Plant Direct PCR kit and when seeding the gel with the PCR product there was a lot of DTT smell. After running the gel, partially degraded dna was observed,...
10 April 2024 2,150 2 View
I am well aware of the methodology for determining lactate threshold; that is not my question. My doubts concern the physiology of lactate transportation to the blood. Once lactate is formed in...
26 February 2024 4,579 0 View
Hi! I want to ask if is there any way to know the weights that the package assigns to each input to make the analysis. If is there any way that would be awesome to my research of forest Regards!
06 November 2023 4,316 0 View
I have GPS locations of animals in which I will use for a resource function analysis. However, this data follows the transects from aerial transect counts and are aligned on lines. I therefore...
19 October 2023 8,438 1 View
I prepared electrocompetents of the AGL1 strain following the "water" protocol: the bacterial culture was resuspended in water, subjected to two washes in water, one in 10% glycerol, and finally...
17 October 2023 4,770 3 View
In FEFLOW specific storage is in fact the compressibility of an aquifer. I have a lake that is part of my model. The units FEFLOW gives for compressibility are 1/m, 1/ft or 10^-4/m. I've searched...
28 November 2022 9,688 3 View
does anyone know how to correct Delauney criterion violations in a FEFLOW mesh in an automatic fashion? And how to automatically perform the equilateralization of triangles after Delauney...
18 November 2021 9,131 1 View
I would really appreciate it if someone direct me to a source that contains scoring information of the CSQ Thank you
28 October 2021 4,781 1 View
Hi, I have a simple question regarding the interpretation of MANCOVA in SPSS. I have found no statistical significance for the overall effect of group according to the multivariate test, but the...
09 September 2021 6,297 6 View
Hi, I am conducting a thematic analysis, and could use an advice what comes to the power and theme prevalence calculation. I have 11 participants with 80% power. I have completed the...
07 September 2021 8,051 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View