Can I use DMEM for AGS human Gastric cancer cell line. As recommended media F-12 media. I found some papers used only DMEM. Is it fine to use it.
Hi Farooq,
I have used AGS cells a lot. If I am correct ATCC recommends F12-K media for AGS. But, with F12K I observed considerable cell death. Therefore, based on number of references I started using RPMI and cells were absolutely fine. Here is the protocol.
GC cell lines AGS (CRL-1739) and KATO-III (HTB-103) cells procured from ATCC were cultured in RPMI1640 (Invitrogen) and F12K (Himedia) media respectively at 370C with 5%CO2 supplemented with 10%FBS, 100U/ml penicillin, 100mg/ml streptomycin (Sigma).
But if you are interested in doing Ca-phosphate mediated transfection then RPMI will be incompatible and you have to change the media from RPMI to DMEM before transfection. References are few (link is provided) for using DMEM media for AGS cells but you can use it. My pervious lab did not observe any problem with AGS cells when used while performing transfection experiments.
http://carcin.oxfordjournals.org/content/21/11/2091.long
Hi Farooq,
I have used AGS cells a lot. If I am correct ATCC recommends F12-K media for AGS. But, with F12K I observed considerable cell death. Therefore, based on number of references I started using RPMI and cells were absolutely fine. Here is the protocol.
GC cell lines AGS (CRL-1739) and KATO-III (HTB-103) cells procured from ATCC were cultured in RPMI1640 (Invitrogen) and F12K (Himedia) media respectively at 370C with 5%CO2 supplemented with 10%FBS, 100U/ml penicillin, 100mg/ml streptomycin (Sigma).
But if you are interested in doing Ca-phosphate mediated transfection then RPMI will be incompatible and you have to change the media from RPMI to DMEM before transfection. References are few (link is provided) for using DMEM media for AGS cells but you can use it. My pervious lab did not observe any problem with AGS cells when used while performing transfection experiments.
http://carcin.oxfordjournals.org/content/21/11/2091.long
I agree with Dr. Jibran Sualeh Muhammad. RPMI1640 is the most recommended medium for AGS. You can propagate AGS and perform experiments with high glucose DMEM or F12, but you have to keep in mid that depending on the medium, the response to the several biological stresses such as redox stress would be totally different. For instance, the amount of cystine and glutamate in the medium affect the activation of system Xc-, which is crucial for the glutathione (GSH) synthesis.
http://jeccr.biomedcentral.com/articles/10.1186/s13046-015-0221-y
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Thanks a lot shafaqat ALi Khan, Jibran Sualeh Muhammad and Go J Yoshida for your scholarly inputs and suggestions.
Could any one tell me, as i culture AGS cells in RPMI-1640, still the cell are floating and they didn't show any growth till 24 hours.
I use DMEM/HIGH GLUCOSE and AGS сells grow well, good luck
L.Garmanchuk
Hello. I'm working with them now. Cells grow well with RPMI640-HIGH GLUCOSE, but they are died in DMEM. Good luck.
could any one suggestion me concentration of Puromycin for AGS cell? what concentration would be excellent?
regards
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