Can I use concanavalin A to stimulate lymphocytes rather than phytohemagglutinin (PHA)?

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Alberto Finamore

yes you ca use ConA to stimulate limphocytes, I usulally use 5microg/mL for 48h

Mohammed Khalid Pasha

Can you please give guide me to a some reference article.

Alla Bucharskaya

Young B. Concanavalin A stimulation of mouse lymphocytes at low concentration. I. The effect of peritoneal exudate cells//Immunology. 1982 May; 46(1): 31–36.

Wands J R , Podolsky D K , and Isselbacher K J Mechanism of human lymphocyte stimulation by concanavalin A: role of valence and surface binding sites//Proc Natl Acad Sci U S A. 1976 June; 73(6): 2118–2122.