I am working on SCE and micronucleus assay. I completed around 30 samples of SCE and MN but suddenly the results get stopped and its been above 1 year struggling with the cell culture experiment. Please contact me at +919416547665 and help me.
Sonal Rajiv Bakshi Mam I am not getting a good metaphase frequency for sister chromatid exchange assay and binucleated cells for micronucleus assay. I am using readymade media (RPMI 1640 Hi-Media).
As discussed on phone - try supplementing with L-Glutamine, refer to the AGT manual for concentration, and also for Tables for troubleshooting regarding metaphase yield. Best wishes!