Reid Selby

5 Questions 1 Answers 0 Followers

Questions related from Reid Selby

Will a thrombin ELISA detect prothrombin levels?

I am interested in measuring the amount of prothrombin and thrombin in the cell culture supernatant of human hepatocytes. Specifically I am interested in this kit from...

02 March 2021 5,955 1 View

Best protocol for automated cell counting?

Hi all, I am performing an exosome quantification assay and I need to normalize my results to total number of cells. I will not be able to detach my cells from the plate, and therefore cannot use...

18 November 2020 6,725 2 View

Do I need collagenase to dissociate hepatocytes?

Hi all, after culturing hepatocytes in a monolayer on collagen in 24 well plates for 5 days, I attempted to trypsinize (0.25% trypsin) them to perform a cell count. However, none of my cells...

12 November 2020 2,696 2 View

Can I use an IHC antibody for ICC?

I am planning a double antibody ICC on hepatocytes using polyclonal antibodies; however, one of the primary antibodies I want to use is only verified for use in IHC. Can I adapt it to be used in...

17 August 2020 4,259 3 View

Do cobalt(II) ions (Co++) interfere with all fluorescent calcium indicators?

Hi all, I would like to measure the effect of hypoxia on free calcium in human hepatocytes. I plan on using cobalt chloride to mimic hypoxia; however, I have read that cobalt ions quench Fura-2...

06 August 2020 1,781 0 View