What is the correct common name for Hydrochoerus hydrochaeris?

There are two extant species of Hydrochoerus: Hydrochoerus isthmius, the lesser or Panamanian capybara, and the genotype species H. hydrochaeris. The latter is the more common species of capybara,...

11 February 2021 3,494 14 View

Is there an alternative to PGLS that considers phylogenetic signal when calculating the estimated value?

I have been working on a study trying to estimate body mass in mammals using a skeletal proxy in R. My dataset has high phylogenetic signal (Pagel's Lambda = 0.9) and I am interested in removing...

26 January 2021 6,997 3 View

Are phylogenetic independent contrasts still used in biology?

I have a regression equation in which I am curious if the amount of scatter around the regression line is driven by phylogeny. I know there is a lot of phylogenetic signal because Pascal's lambda...

22 December 2020 937 1 View

What is the cause of abnormal breathing in anaesthetized rats?

Is anyone able to tell me why my anaesthetized rats often do a 'double breath', or diaphragmatic breath, like a hiccup (see video). This often begins after 1-2 hours under anesthesia, and can...

06 December 2020 2,514 5 View

Best protocol for automated cell counting?

Hi all, I am performing an exosome quantification assay and I need to normalize my results to total number of cells. I will not be able to detach my cells from the plate, and therefore cannot use...

17 November 2020 6,583 2 View

Do I need collagenase to dissociate hepatocytes?

Hi all, after culturing hepatocytes in a monolayer on collagen in 24 well plates for 5 days, I attempted to trypsinize (0.25% trypsin) them to perform a cell count. However, none of my cells...

11 November 2020 2,478 2 View

Problems detecting miR-155 with Advanced Taqman miR kit?

18 October 2020 9,880 3 View

Can I use an IHC antibody for ICC?

I am planning a double antibody ICC on hepatocytes using polyclonal antibodies; however, one of the primary antibodies I want to use is only verified for use in IHC. Can I adapt it to be used in...

16 August 2020 4,073 3 View

Control miRs for RNA extracted from FFPE DLBCL samples?

04 August 2020 1,195 1 View

Can the magnetic permeability and electric permittivity of the vacuum be modeled as the quantum vacuum magnetization and polarization, respectively?

If the magnetic field permeability and electric field permittivity of the quantum vacuum at a point in spacetime may be understood as the effective vacuum magnetization and vacuum polarization,...

04 June 2020 7,655 2 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

How to calculate Total Phenolic Content and express it in mg GAE/kg?

26 January 2021 3,847 7 View

The lipid bilayer broken down and form micelle within 1ns MD simulation. How to simulate lipid bilayer without breaking bilayer?

To simulate the lipid bilayer (10-10, 25-25 so on) from CHARMM-GUI membrane builder, we used to simulate it at various force field (AMBER14, AMBER03, AMBER14IPQ, AMBER15FB, NOVA, YAMBER) at water...

26 January 2021 7,816 2 View

SEM/TEM/AFM which analysis should be performed for spray dried solid lipid nanoparticles?

Can anybody please suggest which analytical technique would give me the best result for the size and shape of solid lipid nanoparticles SEM or TEM or AFM. The nanoparticles are synthesized by...

21 January 2021 7,991 6 View

At what step should one add internal standards in tissue sample for LC-MS?

Hi everyone, I'm doing metabolomics of placental tissue in my lab. I added 1ml of 80% MeOH along with 2 isotopic internal standards namely, phenylalanine and palmitic acid to my sample after...

18 January 2021 9,520 4 View

Someone knows how to preconcentrate bacteria density in water samples?

I have low bacteria density in water samples. I thought to preconcentrate them by filtrating a known volume of water (e.g. 500 ml) in 0.2 um Nylon filters, and then suspended bacteria that has...

14 January 2021 772 2 View

Why the lipid accumulation levels in my 3T3L1 does not match with my adipogenic and lipogenic gene expression levels?

I am using the 3T3-L1 cell line to discover the adipogenic effects of a natural compound and its derivatives. I conducted a lipid accumulation assay using fluorescent Nile Red dye (Adipored) for...

10 January 2021 4,360 1 View

How to approach quantification of cytoplasmic immunofluorescent signal in adipocytes?

Any ideas on how to quantify a non-nuclear immunofluorescent signal in adipocytes? I had the idea to do a watershed on inverted image to "locate" the center of the empty space where lipid droplets...

05 January 2021 5,420 1 View