How can I easily assemble simple sanger DNA sequences into a single sequence?

Note - this is not about Next-gen sequencing or other major deep-sequencing projects I do a lot of simple (cheap) basic sanger sequencing of plasmids with companies like Macrogen and Genewiz....

07 August 2019 5,943 8 View

What percent of Glioblastoma studies use the U87 cell line?

I'm studying glioblastoma and I can't help but notice that a large majority of the basic research papers use this one cell line. Is there a resource anyone knows about that would allow to to see...

01 February 2018 1,249 3 View

How can I clear neuromelanin from a tissue sample?

We are trying to do RNA FISH on a human midbrain sample and are having an issue with auto-fluorescence from the neuromelanin naturally present in the sample. Does anyone have a method to remove...

31 December 2017 4,475 0 View

Anyone have advice for cloning dual-loxp inversion sequences?

I have recently run into a few issues with my loxP constructs. Currently I have a floxed DSRed sequence with Stop codons functioning as a 'stutter' cassette for downstream genes. This way, my...

10 November 2017 4,232 2 View

How can I recover 3D cultures from Matrigel for a phospho-Western Blot?

I am studying vasculogenic potential of cancer stem cells, and so I culture stem cell spheres on Matrigel. After several days, they form 3D tube structures into the gel. Inhibition of my protein...

10 November 2017 251 9 View

How do I minimize oxygen flux when performing routine cell culture tasks on hypoxic cultures?

I am beginning to perform several experiments using cancer cells in a hypoxic environment. Our core facility has a hypoxic incubator that uses nitrogen to displace oxygen down to a minimum of 1%...

09 October 2017 156 4 View

Has anyone differentiated cancer stem cells into vascular endothelial cells?

I have isolated tumor-initiating (cancer stem) cells from a solid tumor and have hypothesized that our molecule of interest mediates the differentiation of these cancer stem cells into...

07 August 2017 3,614 1 View

Anyone have problems with PCR of LoxP inserts?

05 June 2016 3,932 8 View

Adding LoxP stop LoxP to an existing plasmid?

03 April 2016 3,952 7 View

Any advice on cloning a 2A sequence between 2 genes without restriction enzymes?

10 November 2015 4,971 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View