We are trying to do RNA FISH on a human midbrain sample and are having an issue with auto-fluorescence from the neuromelanin naturally present in the sample.
Does anyone have a method to remove or clear neuromelanin from a sample without otherwise destroying the tissue?
I found that methanol and hexane can work in the literature, but I am concerned that this may destroy the silane layers the tissue is attached to, or otherwise compromise the RNA.
Any suggestions?
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