I have a small well 384 plate from IDT with around ~10ul per well. The wells seem to evaporate? at the edges or certainly disappear over time?

Hi This is very odd. Whenever I come back to the plate and defrost it (At room temp for a couple of hours) wells that I know had previously been filled have become void. Some of them I have now...

05 June 2019 9,029 4 View

Can Magnessium Chloride be used to adhere DNA origami to TEM slides?

Hi Can magnessium chloride be used to adhere DNA origamis to TEM slides in the same way it does mica? Im very excited to get new images but im very unfamiliar with the process. Some papers dont...

31 December 2018 8,793 0 View

Im trying to purify gold nanoparticles from DNA in solution via pelleting and resuspending - at what RCF will ~20 base pair DNA begin to pellet?

Im functionalising gold nanoparticles with thiolated DNA, this is done using excess DNA in solution, it is critical that once the procedure is complete any remaining DNA is completely removed....

08 September 2018 7,887 5 View

Cleaning gold nanoparticles by centrifuge. Seems to end up as an aggregated mess?

Hi Im functionalising gold nanoparticles with thiolated oligos. Part of all (Or all that Im aware) protocols require spinning the final solution down at 13,300 rpm for 20 minute cycles to...

10 November 2017 2,542 4 View

Could litmus paper be used to adjust a small ~1ml solution from ~7 to ph 3 then back up to ~7?

I want to functionalise thiolated oligonucleotides with gold nanoparticles. To this I need to adjust the ph of the solution to 3, add Na+, mix, then bring the solution back to 7. I cant be sure...

08 September 2017 183 4 View

Will DNA origami aggregate in purified water or TE buffer?

Hi Hollow DNA spheres. Im not getting a good number to stick to mica for imaging and they seem to be aggregating when I try and get a size distribution of them in a zetasizer. Im trying to use a...

07 August 2017 3,247 2 View

As oppose to cutting out bands on an electrophoresis gel would a size exclusion column be a effective way to seperate DNA origami from excess staples?

04 May 2017 1,255 2 View

Matlab script to define N Z Mu and Epsilon from HFSS scatter parameters from a C resonator in the optical wavelength spectrum?

Hi I am having trouble accurately finding the refractive index of a structure I simulate in HFSS. I have been using chapter 3 of optical metamaterials (Wenshan Cai Vladimir Shalaev) to aid me in...

03 April 2017 633 2 View

Advice regarding SEM of DNA origami seeded with amine bound nanogold?

Hi I'm not having any success (Nothing visible on SEM) binding amine bound 1.4nm nanogold to DNA origami structures. I'm currently using a 100Kd protein concentration column, where origami and...

02 March 2017 1,004 4 View

Thoughts on functionalising gold nanoparticles with synthetic oligonucleotides for site specific attachment in to a larger DNA structure?

Having conducted global aggregation of gold to DNA origami and acheived some success I am now interested in pursuing site specific attachment. I am looking through the literature and have found...

02 March 2017 7,852 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View