I have a small well 384 plate from IDT with around ~10ul per well. The wells seem to evaporate? at the edges or certainly disappear over time?

Hi This is very odd. Whenever I come back to the plate and defrost it (At room temp for a couple of hours) wells that I know had previously been filled have become void. Some of them I have now...

05 June 2019 9,029 4 View

Im trying to purify gold nanoparticles from DNA in solution via pelleting and resuspending - at what RCF will ~20 base pair DNA begin to pellet?

Im functionalising gold nanoparticles with thiolated DNA, this is done using excess DNA in solution, it is critical that once the procedure is complete any remaining DNA is completely removed....

08 September 2018 7,887 5 View

Cleaning gold nanoparticles by centrifuge. Seems to end up as an aggregated mess?

Hi Im functionalising gold nanoparticles with thiolated oligos. Part of all (Or all that Im aware) protocols require spinning the final solution down at 13,300 rpm for 20 minute cycles to...

10 November 2017 2,542 4 View

Could litmus paper be used to adjust a small ~1ml solution from ~7 to ph 3 then back up to ~7?

I want to functionalise thiolated oligonucleotides with gold nanoparticles. To this I need to adjust the ph of the solution to 3, add Na+, mix, then bring the solution back to 7. I cant be sure...

08 September 2017 183 4 View

Can I use a PCR purification column to remove TCEP from a sample of reduced, thiolated, ssDNA sequences?

Can a PCR purification column be used in this manner? Thanks

08 September 2017 5,492 3 View

Will DNA origami aggregate in purified water or TE buffer?

Hi Hollow DNA spheres. Im not getting a good number to stick to mica for imaging and they seem to be aggregating when I try and get a size distribution of them in a zetasizer. Im trying to use a...

07 August 2017 3,247 2 View

As oppose to cutting out bands on an electrophoresis gel would a size exclusion column be a effective way to seperate DNA origami from excess staples?

04 May 2017 1,255 2 View

Matlab script to define N Z Mu and Epsilon from HFSS scatter parameters from a C resonator in the optical wavelength spectrum?

Hi I am having trouble accurately finding the refractive index of a structure I simulate in HFSS. I have been using chapter 3 of optical metamaterials (Wenshan Cai Vladimir Shalaev) to aid me in...

03 April 2017 633 2 View

Advice regarding SEM of DNA origami seeded with amine bound nanogold?

Hi I'm not having any success (Nothing visible on SEM) binding amine bound 1.4nm nanogold to DNA origami structures. I'm currently using a 100Kd protein concentration column, where origami and...

02 March 2017 1,004 4 View

Thoughts on functionalising gold nanoparticles with synthetic oligonucleotides for site specific attachment in to a larger DNA structure?

Having conducted global aggregation of gold to DNA origami and acheived some success I am now interested in pursuing site specific attachment. I am looking through the literature and have found...

02 March 2017 7,852 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

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02 March 2021 932 1 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

No title

01 March 2021 3,905 5 View

How Download/Extract citations of 1 given year ALL my publications in Google Scholar?

If you google this you find a lot of smart guys explaining you how to download 1 year of citations of 1 single paper of yours (or anyone else). I mean 1 year of ALL my papers. or ... ALL years or...

01 March 2021 1,121 9 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View