As oppose to cutting out bands on an electrophoresis gel would a size exclusion column be a effective way to seperate DNA origami from excess staples?

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I have a small well 384 plate from IDT with around ~10ul per well. The wells seem to evaporate? at the edges or certainly disappear over time?

Hi This is very odd. Whenever I come back to the plate and defrost it (At room temp for a couple of hours) wells that I know had previously been filled have become void. Some of them I have now...

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Can Magnessium Chloride be used to adhere DNA origami to TEM slides?

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Im trying to purify gold nanoparticles from DNA in solution via pelleting and resuspending - at what RCF will ~20 base pair DNA begin to pellet?

Im functionalising gold nanoparticles with thiolated DNA, this is done using excess DNA in solution, it is critical that once the procedure is complete any remaining DNA is completely removed....

08 September 2018 7,887 5 View

Cleaning gold nanoparticles by centrifuge. Seems to end up as an aggregated mess?

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10 November 2017 2,542 4 View

Could litmus paper be used to adjust a small ~1ml solution from ~7 to ph 3 then back up to ~7?

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Can I use a PCR purification column to remove TCEP from a sample of reduced, thiolated, ssDNA sequences?

Can a PCR purification column be used in this manner? Thanks

08 September 2017 5,492 3 View

Will DNA origami aggregate in purified water or TE buffer?

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Matlab script to define N Z Mu and Epsilon from HFSS scatter parameters from a C resonator in the optical wavelength spectrum?

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Advice regarding SEM of DNA origami seeded with amine bound nanogold?

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Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

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New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

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Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

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Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

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Aamal Ghazi Mahdi Al-Saadi

Yes, give it a try.

Good luck

Paul Rutland

Many fillings would work but sephadex g75 0r G100, superdex 200 and sepharose 6B all fall within the useful size range depending a little on the shape of the molecule but any matrix that includes the size 450kd and includes 45kd should work