I have found primers from our research group for a particular gene of interest but am not sure of the fragment size. Can I predict this from the primer sequence?
Use the Primer-Blast
http://www.ncbi.nlm.nih.gov/tools/primer-blast/
of course you can find the exact size of the PCR fragment you would get out of the primer pair you have, provided the primer is really highly specific for the gene you are interested in. You will have to get the sequence from the database and just try to find where your primer binds in the sequence. Mark those positions and count the spanning region!
There could be another problem if your gene of interest has splice variants and if the primer is not specific for only one variant, in that case you will have to check againts all the variants if your primer is specific or not.
Hope this helps.
Good luck!
Use both F & R primer sequence joined together and use it as query in NCBI blast search. Use just blast (somewhat similar similar sequence) not mega blast (default in ncbi blast). if it hits multiple sequences , look for exact matches
You can predict fragment size using primer Blast on NCBI and can also be estimated by programs like fastPCR which is freely available online
If its genomic DNA you can use this programm
http://genome.ucsc.edu/cgi-bin/hgPcr?command=start
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