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Questions related from Navjyoti Chakraborty
I performed silver staining using a standard protocol. But the problem is As soon as I add silver nitrate to gel, the solution turns white and the gel becomes white. What could be the reason for...
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I am trying to do some stress related assays on plants and have limited amount of tissue. When searching literature for catalase assay, I found protocols where tissues are used for doing catalase...
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I ran a 15% SDS-PAGE gel. After the end of the run the lower bands of the marker/ladder were visible clearly. But when the gel was stained with commassie stain, the lower half of the bands in the...
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I am trying to express a plant membrane protein using pGEX vector in BL21 strain. I could express the protein but all went into the pellet fraction. Should I purify the protein from the pellet...
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mbSUS has been used successfully by several researchers to screen for protein-protein interaction for membrane proteins but I could not find if anyone has used it to screen cDNA library. if it is...
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