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Questions related from Navjyoti Chakraborty
I have a plant whose roots have been infected with both bacteria and fungi. What strategy should I follow to isolate RNA of all three, i.e. plant, bacteria and fungi at once? This RNA is to be...
10 June 2024 9,903 0 View
I performed silver staining using a standard protocol. But the problem is As soon as I add silver nitrate to gel, the solution turns white and the gel becomes white. What could be the reason for...
15 September 2015 8,183 4 View
I am trying to do some stress related assays on plants and have limited amount of tissue. When searching literature for catalase assay, I found protocols where tissues are used for doing catalase...
09 January 2015 6,495 4 View
Can anyone suggest which physiological and genetic factors/genes affect the number of siliques in Arabidopsis thaliana?
10 October 2014 2,561 5 View
I ran a 15% SDS-PAGE gel. After the end of the run the lower bands of the marker/ladder were visible clearly. But when the gel was stained with commassie stain, the lower half of the bands in the...
03 September 2014 1,661 8 View
I am trying to express a plant membrane protein using pGEX vector in BL21 strain. I could express the protein but all went into the pellet fraction. Should I purify the protein from the pellet...
13 February 2013 5,677 13 View
mbSUS has been used successfully by several researchers to screen for protein-protein interaction for membrane proteins but I could not find if anyone has used it to screen cDNA library. if it is...
07 November 2012 6,363 1 View
