PDX leukemia in immunodeficient mice show regression after a time. Anyone knows what is going on?

I am performing PDX ALL in NSGS mice, but something curious happens: some leukemias develop very well , but after a time the hCD45+ cells show a huge regression while the mCD45+ cells turn back to...

16 December 2020 7,344 2 View

About the mouse humanization process, why should I wait between four to 24 hours after irradiation to transplant the stem cells into pups?

The protocol for the humanization of the NSG mice with human CD34+ cells demands that mice should be irradiated before the inoculation of the human cells. However, after this procedure, it is...

01 September 2020 8,477 4 View

How to calculate dielectric constant from oil spill in SAR satellite image?

I'm trying to understand how to determine relationship between reflectivity in SAR images and dieletric constant of oil spill offshore registred in image.

17 August 2020 2,094 2 View

What is the best methodology to identify and to quantify different classes of lipids from pollen?

I would like to characterize and quantify different classes of lipids of different samples of pollen. I was thinking to use HPLC or GC-MS. Someone know if these techniques are good for that?...

09 April 2018 3,379 4 View

Xenograft of DND-41 cell line in NUDE mice.

I am trying to develop subcutaneous xenograft model in NUDE mice, using DND-41 tumor cells. I already tried sometimes, without success. I am using 100% and/or 50% of matrigel.Someone knows if is...

22 February 2016 7,994 8 View

There is some special condition for culturing BT474 breast cancer cell line?

I am used to work with BT474 cell line. But as I was needing a huge amount of cells, I expanded them in 4 flask T150 during 3 weeks. It is known that the growth is very slow, but suddenly, when...

22 February 2016 2,431 5 View

Do you know any software that can analyze absence/presence dataset from allopolyploid microsatellite data?

12 October 2015 1,282 4 View

Can I supplement the culture medium with mouse serum instead of bovine serum to culture the cells?

13 February 2014 4,745 2 View

How to determine an antibody titer in a Whole Cell ELISA?

I am trying to get a monoclonal antibody against a single transmembrane protein. I am using the Whole Cell ELISA to test the immunized mouse serum. The serum is tested against the cell with or...

02 September 2013 2,649 13 View

How obtain stable transfect Hek293T cell line using blasticidin to selection?

I tried to stably transfect Hek293T with linearized pEF6/V5 plasmid, that contain the blasticidin resistance gene. The negative control cells (no-transfected Hek293T) died into about one week. I...

04 October 2012 214 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View