Can I mix LB agar with simple LB to make the top agar(for double agar method)?

More Oana Izmendi's questions See All

Does anyone have an idea/procedure on cleaning/sterilizing a plexiglass photobioreactor?

I'm interested in your previous experience with growing diatoms in plexiglass PBRs. I intend to use the photobioreactor for monospecific cultures/experiments with Skeletonema costatum. It has a...

29 May 2023 7,925 3 View

Have you ever encountered technical issues with the sensors embedded by Emotiv Insight EEG Headset and was it necessary to dissasemble or repair it?

By technical issue, I mean a sensor (for example AF4) that is gray (no electrical contact) so that the Emotiv Insight may have an internal broken wire. I followed the YouTube Tutorial...

25 August 2022 8,154 0 View

Is there anyone who tried to use BCI2000 to acquire the raw EEG signal detected by the embedded sensor of the NeuroSky portable headset?

I am interested in finding out if the BCI2000 software platform is still applicable for the newest NeuroSky EEG portable headset released in the year 2018.

06 August 2021 8,962 1 View

Could you please suggest to me some online databases of EEG datasets aimed at recording the voluntary eye-blinks?

Currently, I am a Ph.D. candidate pursuing the development of innovative projects related to Biomedical Engineering and Mechatronics fields. In the last 7 years, I was deeply involved in the...

25 July 2021 7,237 2 View

What are the most effective tools for informing-raising awareness of local communities in a protected area?

Based on your experience, what tools do you consider the most effective in the information and awareness activities of the communities in the area of a natural park: public debates, informative...

13 June 2021 8,115 3 View

Qualitive Research Data : How to make use of Youtube?

I am currently researching for my Master's Thesis (the subject is the redefinition of marriage in Korea). At first, I wanted to use detailed interviews as my qualitative research data. But with...

30 May 2021 4,455 7 View

Dependence of FWHM of Bragg peaks on sample tilt?

For a series of single crystal thin films (thicknesses between T=20-300 nm) I observe a narrowing of the (444) Bragg peak with increasing T, as expected from the Sherrer formula considering the...

03 August 2020 9,619 2 View

Which prospective observational studies in the field of diabetes do you know?

I am looking for large prospective observational studies for patients with diabetes. No interventions. Just longitudinal assessment. Thank you!

22 September 2019 5,222 2 View

What are the best tools to collect data from social media?

what are the best free tools/programs/apps to collect data from social media?

02 March 2017 4,826 10 View

Could anybody tell me how many international tourism promotion campaigns have been developed in Bulgaria in the last 25 years?

I'm interested in international campaigns developed by the Bulgarian national tourism authorities.

02 February 2016 9,080 3 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Recovery Viurses from bacteria genome?

Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...

28 July 2024 8,953 1 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

Should all lactic acid bacteria be catalase negative? should all probiotic bacteria be catalase negative?

I am working on probiotic bacteria for fish, and I am trying to isolate probiotic bacteria like lactic acid bacteria or other probiotics from the gastrointestinal tract of fish using different...

12 July 2024 3,756 4 View

Can we change blood agar in the mCIM and eCIM protocols?

In the modified carbapenem inactivation method (mCIM) and EDTA-carbapenem inactivation method (eCIM) protocols, can we replace blood agar with another culture medium?

10 July 2024 6,352 0 View

Arabidopsis contamination in Hydroponic Culture ?

Hi I have a real problem growing Arabidopsis in hydroponic culture .I am using the bvoxes from Araponics and a Binder growth chamber with short day conditions and everything accurate .the seeds...

25 June 2024 9,151 6 View

Does broad spectrum phage become narrow spectrum by time ?

In the host range analysis, I saw that a temperate phage that I isolated had an effect against a different strain, but later (2 years) it did not affect that strain. What could be the explanation...

25 June 2024 9,729 3 View

Why is my phage library obtained at round 2 of Biopanning viscous?

After incubation of the TG1 cells with the helper phage, I spun down the culture and precipitated the supernatant with PEG/NaCl. After this, I spun it down again and resupended the pellet in PBS....

21 June 2024 6,260 2 View