What is the accurate and simplest method for detection bacterial siderophore?

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Can we use Cu+2 instead of Fe+3 in Blue Agar CAS Assay for siderophore detection?

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Can I use CAS (Chrome Azural S) assay to measure siderophore production in iron containing media?

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Isolation of siderophore producing bacteria ?

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Hamza Ettadili

you could make a qualitative assay of siderophores production for the selected bacteria using LB or any other agar plate medium containing Chrome azurol S (CAS), which turns blue when bound to Fe and reverts to yellow/orange being released(case of bacteria which produce siderophores).

Mohammed A.S Issa

Thank you m dear Hamza Ettadili for our answer

Phil Geis

https://accuplexdiagnostics.com/siderotec-assay-range/