What can explain a low expected heterozygosity rate for T. monococcum wild samples (0.09) ?

I conducted an SDS PAGE electrophoresis on wheat wild varieties to separate their glutenins and measure the genetic diversity of my sample. I only ran one electrophoresis. Can a genetic drift...

14 February 2021 3,971 1 View

Is there any evidence that using ball milling in intervals, rather than one long run, improves the mixing process?

I am trying to synthesize nanoparticles by mixing the precursors by ball milling. I would like to know if mixing them for one hour straight is less or more efficient than mixing them in intervals...

10 November 2020 8,527 9 View

Can I measure serum and not tissue succinate dehydrogenase?

Dear Colleagues I am interested in evaluating the concentration of SERUM succinate dehydrogenase in a certain type of cancer. I found an ELISA kit for it however, I can barely find any papers...

16 October 2020 547 1 View

How to monitor and mapping crisis event tweets based on my tweets dataset?

how to monitor and mapping crisis event tweets based on my tweets dataset. I need tools for that. I was classified as tweets to damaged and not. then I have extracted locations from textual...

18 August 2020 4,068 2 View

Why Matrix Inverse on Python Gives Different Solution from Excel?

When I compute matrix inverse on Python, it gives me slightly different results from Excel (difference appears after the third or forth number, however it highly affects my work). I know that for...

01 August 2018 2,466 9 View

How can I fluorescently label graphene oxide to be able to see it under confocal microscope?

I have tried to fluorescently label graphene oxide sheets once with Fluorescein sodium salt and another time with Fluorescein isothiocyanate (FITC) by mixing 500 uM of Fluorescein (any of them)...

24 September 2017 1,449 3 View

Can NS2 be used to calculate the memory usage and computation time of a particular agent inside the network node?

I added a security agent to the NS2 core. My aim is to calculate the extra overhead (time and memory allocation) due to the addition of this agent inside the network nodes. Is that possible using...

26 July 2017 7,974 2 View

Is anyone interested in collaborating on this project?

09 July 2015 1,333 15 View

By what method can i improve the learning of neural network?

Learning of neural network

08 November 2011 9,139 3 View

Python code for training Arabic spacy NER model not giving result or errors.

This is the code to train the spacy model for NER. My dataset is Arabic tweets JSON file. I tagged location manually labeled in my dataset by https://dataturks.com machine learning tools but the...

01 January 1970 6,110 1 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How we can correct the slide?

i am doing research on E.feacalis that is grown in dentin and after that, we did a gram stain for the sample my Q first does the gram stain consider as reliable tool for testing and seeing the...

14 February 2021 6,697 1 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

Review Papers regarding transcriptional regulations & promoter types/classes in Gram positive bacteria?

Dear fellow researchers, Could you please suggest me excellent review papers discussing transcriptional regulations and/or promoter classes in Gram positive bacteria? Thank you so much for your help!

08 February 2021 6,909 3 View

Flexibility of RNAseq?

When you use RNAseq I understand that it must follow certain conditions when you use it. One of them is the insertion of a polyA tail. The insertion of the polyA must include then...

05 February 2021 3,770 4 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

Moving bacteria-like organisms in my HEK293T cell culture?

Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...

02 February 2021 7,892 4 View