Does it change based on the incubation period of the drug with the cells 48hrs versus 72 hrs ?
Is it like MNPT (geometrical mean of at least 20 healthy subjects) ? or it's the arythmetical mean ? can a pool of healthy subjects' plasma be considered as the reference APTT for calculating APTT...
01 April 2024 1,846 0 View
Hello everyone, Does anyone know If I want to represent a DNA sequence from Snapgene viewer in the manuscript to point the location of a specific amino acid how I can do that? Thanks
09 January 2024 9,030 3 View
What value of the OD at 600nm corresponds to a bacterial concentration of the order of 10*8 CFU/ml for Gram-positive bacteria?
27 October 2023 9,521 5 View
I want to calculate the electronic properties and determine the effect of U parameter on the gap energy
14 June 2023 8,987 2 View
I have a research where I have several (Check all that apply questions) and of course each question has multiple answers. I'm confused about the best way to analyze them. as there are a lot of...
30 May 2023 5,322 3 View
If all my dependent and independent variables are stationary at first difference NOT at level, SHOULD I REPLACE (ardl y x1 x2) with (ardl d.y d.x1 d.x2)? when there is no cointegration and I want...
14 April 2023 3,377 7 View
I am testing the IC50 of cisplatin ( dissolved in DMSO) on MCF7. I am not getting a response even at high doses Of the drug.
05 April 2023 8,390 3 View
I am a PhD student in chemistry; And I’m working on “Analytical study of Borago officinalis L., in view of its valorization in the therapeutic field”, The training will help me realize part of my...
01 March 2023 6,632 1 View
I want to carry out a hydroponic culture with a simplistic device that can be carried out in a laboratory of wheat seeds to which I wish to subject salt stress and bacterial inoculation in order...
01 February 2023 694 5 View
Hello scientists, I was hesitant to ask such a question because it is somewhat simple, but in the end there is no shame in the learning process, so, What consumes more energy, the polymerization...
05 January 2023 1,797 3 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
My case has been under treatment now for 3 years with little response. Now considering euthanasia. Last ditch is Cisplatin for dilution in iv infusion (100ml/ml in saline and mannitol). What can I...
05 August 2024 1,835 0 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
I am working in the filed of long acting drugs with Nano formulation. I want to study the efficacy of my formulation in 2D cell cultures while this required media change on every two days. In such...
24 July 2024 8,696 0 View
Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...
19 July 2024 2,155 0 View
How to calculate percentage cumulative drug release when, Total volume of dissolution media = 250ml Added formulation was equivalent to 10mg of drug Sample withdrawn = 1ml each time Sink condition...
15 July 2024 4,192 1 View
Hi, In the 6 well plate, when I am seeding the cells. Do I add 1ml of complete media to one of the wells for the control at this stage or do I wait unit I am treating the plates to do that? Also...
13 July 2024 9,783 1 View
In my country, there is a lot of public outcry in relation to the presence of drug injection and related behaviors in public. Hence at least the bigger cities would probably benefit from the...
08 July 2024 8,903 1 View
Hello everyone reading this topic! It is well known that microcrystalline cellulose 102 is recommended for use in direct compression technology, however, like other grades, it has poor flowability...
08 July 2024 2,807 0 View