Dear All
My target is to use natural solvent that free toxin such as water in extraction active compound from dragon fruit peel. the active compounds such as phenolic content and mineral profile. These active compound can be used as additive in natural food product. what criteria should I consider if I use water as a solvent into microwave assisted extraction? An addition water gave the highest value TPC and antioxidant activity as description below (Lourith and Kanlayavattanakul, 2013). In this experiment I try to avoid using ethanol because I believe if use water don't need for purification process after extraction such as rotary evaporator.
solvent:Ethanol
TPC (mgGAE/g extract)=1.193+/-0.011
DPPH (ug/ml)=823.580+/-13.250
solvent:Water
TPC (mgGAE/g extract)=1.351+/-0.021
DPPH (ug/ml)=261.520+/-0.980
Dear Azlan Nazeri,
The best way is to compare the TPC and DPPH activity of ethanol and water extract of your samples, according to the obtained results You can select the conditions of extraction.
Best Regards,
Vesna
I would suggest you to perform quick extraction with hydro-alcoholic mixture (start with 80:20 = ethanol: water). Check for TPC and DPPH and compare the results with pure solvents (water and ethanol).
Additionally, you should calculate the yield of extraction using aforementioned solvent systems.
Best wishes!
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