Can I incubation the cell for 6h outside of CO2 incubator?

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Sven Reister

Hi,

you could use a hepes buffered medium as this is not CO2 dependent. Regarding Temperature: Perhaps then you could use a normal heating cabinet or so

Best Sven

José Luis Esteban

The Petaka bioreactor from Celartia self-regulates internal CO2 (http://petaka.com/no-need-for-co2) so you only need to maintain temperature control.

Ron Hrstka

Can you put a small shaker into your incubator? I have done this before.