Due to Coronavirus we have to urgently freeze or throw out our cells mid-reprogramming (CytoTune-iPS 2.0 Sendai Reprogramming). Has anyone ever done this and have any recommendations?
Many thanks!
I routinely freeze at day 7 when they still have sufficient fibroblast-like characteristics that they survive normal fibroblast freezing methods just fine. I have done this with hundreds of reprogrammed lines and never had a failure.
Unfortunately, I never have had the need to try freezing them at a later stage and so I cannot speculate as to later survival.
Hi,
I dont think there are any yes or no answers here.
In general, if the option is to throw them away, then I'de say Ice 'em. Efficiency will likey drop depending on at what stage they are. But I would expect some to survive.
Naturally if you are studying the actual reprogramming they might not be very useful for comparisons to other lines.
Make sure to seed them at high density when thawing!
Good luck.
Best, Staffan
If the freezing is done by day 7, there is a definite yes or no answer, and that answer is yes. Survival and efficiency are exactly the same as reprogrammings that were not frozen and they are plated at exactly the same density.
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